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The frequency of N 2 O use was half a whipped cream propellant cartridge (approximately 7.0 liters of N 2 O may be released from each cartridge) daily for the last 2 years, and 1 month before admission, this had increased to 1 propellant cartridge daily
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RNA-seq For MEFs 1–3 The concentration of total RNA extractions was quantified with the Nanodrop One (Thermo Fisher), and RNA integrity was assessed with the Bioanalyzer 2100 RNA Nano assay (Agilent)
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