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Culture and transient transfection of 293T cells 293T cells were routinely cultured in DMEM (Sigma) supplemented with 10% fetal bovine serum and penicillin and streptomycin
firming serum
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In all experiments cell death was quantified using the scalable time-lapse analysis of cell death kinetics (STACK) imaging method (Forcina et al, 2017)
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