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Similar content being viewed by others Introduction Biological thiols, including glutathione (GSH), cysteine (Cys) and homocysteine (Hcy), play a pivotal role in many biological processes such as regulating redox homeostasis, detoxification and metabolism and as an active site of enzymes and cofactors 1,2
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In the latter regard, it is important to emphasize that experimental outcomes can be influenced by factors beyond the accuracy of structure annotation, including metabolite instability or degradation in tissue, low endogenous concentrations leading to low-quality MS/MS spectra, and matrix effects in biological samples
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