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Role of glutathione peroxidase 1 in glucose and lipid metabolism-related diseases - ScienceDirect

2.3.3.4 Ascorbate peroxidase activity The APX activity was determined by adding 100 µL of the extract into 2.7 mL of the reaction medium containing potassium phosphate buffer (50 mM,pH 6) and ascorbic acid (0.8 mM).The reaction started by adding 200 µL of hydrogen peroxide H 2 O 2 (2 mM)

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1) and is initiated by the activation of SREBP1 [17, 18]