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Analyze the quantity and quality of your purified protein by loading approximately 20-40 μl of each fraction with a standard BSA concentration on a SDS-PAGE and stain with Coomassie blue ( Figure 3B ) or SYPRO protein stain for visualization
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Using an in vitro medium, butyrate and propionate treatment was shown to improve intestinal cell proliferation in human biopsy samples [134]
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