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While it appeared that mutating any site (C, N A or N B ) affected the binding to GABARAP, the strongest effects were observed with the ∆C N B mut and ∆C ∆N A N B mut combinations, both increasing the Kd for GABARAP from 1.8 µM for the wild type ATG14-BECN1 heterodimers to about 28 µM, which likely corresponds to a non-specific interaction with GABARAP

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Recently, investigators developed an innovative device that allows in vivo microdialysis to simultaneously sample brain alcohol, acetaldehyde, and acetate while animals are ingesting alcohol (150)